Understanding Peptide Purity, HPLC Testing & COAs
An accessible guide to analytical purity, HPLC, mass spectrometry, Certificates of Analysis and the distinction between purity, identity and sterility.
What Does Peptide Purity Mean?
Purity describes the proportion of analysed material attributed to the desired component under a stated method; it is not necessarily peptide content by mass.
HPLC & Mass Spectrometry
HPLC can assess chromatographic composition, while mass spectrometry can provide molecular-mass evidence. Neither method alone establishes sterility or safety.
What Is a Certificate of Analysis?
A COA reports selected information and results for a specified material or batch, including identifiers, test dates, methods and reported results where available.
Purity vs Identity
Purity and identity are related but distinct analytical questions. Complementary methods can provide a more complete picture.
Purity Does Not Mean Sterility
A high analytical purity percentage is not proof that a product is sterile, safe for administration or suitable for human use.
Applicable research products are intended for laboratory and research purposes only and are not for human or animal consumption.